Translation examples
Viime aikoina on onnistuttu tuottamaan puskureita, joiden avulla proteiinit voidaan erotella kysteiinin pKa:n alle menevässä pH:ssa (esimerkiksi bis-tris, pH 6,5) ja sisällyttää erotteluun pelkistintä (esimerkiksi natriumbisulfaatti).
Recent advances in buffering technology alleviate this problem by resolving the proteins at a pH well below the pKa of cysteine (e.g., bis-tris, pH 6.5) and include reducing agents (e.g. sodium bisulfite) that move into the gel ahead of the proteins to maintain a reducing environment.
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