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Esempi di traduzione.
Pellet was resuspended in buffer (20 mM HEPES, 5 mM EDTA, 2 mM DTT) and disintegrated by sonification with the ultrasonic processor UP400S (application of energy ∼ 400 Ws).
Pelletti suspendoitiin uudelleen puskuriin (20 mM HEPES, 5 mM EDTA, 2 mM DTT) ja hajotettiin sonifioimalla ultraääniprosessorilla Up400s (energian käyttö ~ 400 Ws).
Recent advances in buffering technology alleviate this problem by resolving the proteins at a pH well below the pKa of cysteine (e.g., bis-tris, pH 6.5) and include reducing agents (e.g. sodium bisulfite) that move into the gel ahead of the proteins to maintain a reducing environment.
Viime aikoina on onnistuttu tuottamaan puskureita, joiden avulla proteiinit voidaan erotella kysteiinin pKa:n alle menevässä pH:ssa (esimerkiksi bis-tris, pH 6,5) ja sisällyttää erotteluun pelkistintä (esimerkiksi natriumbisulfaatti).
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